分子量:104 kDa

纯度:>85% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the HCMV Glycoprotein B (gB), Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:

产品特性:HCMV Glycoprotein B (gB), Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 104 kDa. The predicted N-terminus is Val 23. The reducing (R) protein migrates as 140-150 kDa in SDS-PAGE  due to glycosylation.

产品背景:Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.